|
KMID : 0613820000100010045
|
|
Journal of Life Science
2000 Volume.10 No. 1 p.45 ~ p.51
|
|
|
Ovarian Development and Yolk Protein of the Mushroom Fly, Coboldia fuscipes (Diptera: Scatopsidae)
|
|
|
|
|
Abstract
|
|
|
|
Ovarian development and yolk protein (YP) of mushroom fly, Coboldia fuscipes, were characterized. C. fuscipes has a pair of ovaries, composed of 130∼140 ovarioles, respectively. Ovarian development begins at 1 day of pupa, and growth of the ovaries continued to become a matured shape at 1 day after emergence. The YP of C. fuscipes identified by SDS-PAGE analysis revealed that the protein is composed of three subunits, designated YP1 (61 kDa), YPS(50 kDa), and YP3 (47 kDa). These three subunits of YP gradually decreased during embryogenesis. The YP was first detected in the 3 day-old pupal ovary and was continually detected up to 2 day-old adult, but not in the hemolymph. Furthermore, Western blot analysis of male and female adult hemolymph and ovary revealed that the antibodies against YP1, YP2, and YP3 reacted with three YP bands in ovary and egg extract, respectively. However, this reactivity was not observed in the male and female hemolymps. Therefore, it is assumed that the UP of C. fuscipes is synthesized in the ovary at 3 days after pupation.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|